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. 2014 May 9;9(5):e96765. doi: 10.1371/journal.pone.0096765

Figure 2. Oncogenic receptor expression and activation are regulated by miR-133a in lung cancer cell lines.

Figure 2

(A) The target genes of miR-133a were determined from Targetscan prediction software and the two database containing mRNA expression levels of receptors in miR-133a-expressing cells. (B) Co-transfection of CL1-0 cells with the AS2-Neo vector (Ctl) or AS2-Neo-miR-133a-expressing plasmid with firefly luciferase fused with 3′UTR sequences of putative miR-133a target genes. Luciferase activity was measured, and the relative ratio of the activity in the miR-133a groups to that in the control vector group is presented. (C) 72 hours after transient infection with the miR-133a-expressing virus, the protein expression levels of receptors in CL1-5 and A549 cells were determined. (D) The phosphorylation level of IGF-1R was determined in CL1-5/AS2-Neo- or CL1-5/miR-133a-expressing cells by the Phospho-Receptor Tyrosine Kinase Array.