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. 2014 May 9;9(5):e96757. doi: 10.1371/journal.pone.0096757

Table 2. Plasmids.

Plasmid Relevent Genotype or Description Reference or Derivation
pET19b T7 promoter expression vector, AmpR Novagen
pSKH001 pET19b Encoding N-terminal hexahistidine tagged B. subtilis BirA, AmpR This study
pET28b T7 promoter expression vector, KanR Novagen
pSKH003 pET28 encoding accB-86 This study
pSKH004 pET28 encoding pyc-77 This study
pQC026 pET28b encoding C-terminal Hexahistidine tagged BLAP [23]
pBAD322K Medium copy expression vector, KanR [48]
pSKH005 pBAD322K encoding B. subtilis 64-325 This study
pSKH006 pBAD322K encoding B. subtilis WT BirA This study
pSKH007 pBAD322K encoding E. coli BirA 66-321 This study
pSKH008 pBAD322K encoding E. coli WT BirA This study
pSKH009 pBAD322K encoding B. subtilis 66-325 This study
pSKH010 pBAD322K encoding B. subtilis 75-325 This study
pSKH011 pBAD322K encoding B. subtilis 82-325 This study
pBAD322Cm Medium copy expression vector, CmR [48]
pSKH012 pBAD322Cm encoding B. subtilis 64-325 This study
pSKH013 pBAD322Cm encoding B. subtilis WT BirA This study
pSKH014 pBAD322Cm encoding E. coli BirA 66-321 This study
pSKH015 pBAD322Cm encoding E. coli WT BirA This study
pSKH016 pBAD322Cm encoding B. subtilis 66-325 This study
pSKH017 pBAD322Cm encoding B. subtilis 75-325 This study
pSKH018 pBAD322Cm encoding B. subtilis 82-325 This study
pSKH019 pET19b encoding N-terminal hexahistidine tagged BirA 64-325, AmpR This study
pSKH020 pET19b encoding N-terminal hexahistidine tagged BirA 66-325, AmpR This study
pSKH021 pET19b encoding N-terminal hexahistidine tagged BirA 75-325, AmpR This study
pSKH022 pET19b encoding N-terminal hexahistidine tagged BirA 82-325, AmpR This study
pMUTIN4 spoVG-lacZ, ermR [49]
pDR111 SpcR, 5'-amyE, 3'-amyE, phyper-spank G. W. Ordal
pSKH023 pMUTIN4 encoding B. subtilis bioW internal 500 bp fragment This study
pSKH024 pDR111 encoding B. subtilis accB-86 This study