pET19b |
T7 promoter expression vector, AmpR |
Novagen |
pSKH001 |
pET19b Encoding N-terminal hexahistidine tagged B. subtilis BirA, AmpR |
This study |
pET28b |
T7 promoter expression vector, KanR |
Novagen |
pSKH003 |
pET28 encoding accB-86 |
This study |
pSKH004 |
pET28 encoding pyc-77 |
This study |
pQC026 |
pET28b encoding C-terminal Hexahistidine tagged BLAP |
[23]
|
pBAD322K |
Medium copy expression vector, KanR |
[48]
|
pSKH005 |
pBAD322K encoding B. subtilis 64-325 |
This study |
pSKH006 |
pBAD322K encoding B. subtilis WT BirA |
This study |
pSKH007 |
pBAD322K encoding E. coli BirA 66-321 |
This study |
pSKH008 |
pBAD322K encoding E. coli WT BirA |
This study |
pSKH009 |
pBAD322K encoding B. subtilis 66-325 |
This study |
pSKH010 |
pBAD322K encoding B. subtilis 75-325 |
This study |
pSKH011 |
pBAD322K encoding B. subtilis 82-325 |
This study |
pBAD322Cm |
Medium copy expression vector, CmR |
[48]
|
pSKH012 |
pBAD322Cm encoding B. subtilis 64-325 |
This study |
pSKH013 |
pBAD322Cm encoding B. subtilis WT BirA |
This study |
pSKH014 |
pBAD322Cm encoding E. coli BirA 66-321 |
This study |
pSKH015 |
pBAD322Cm encoding E. coli WT BirA |
This study |
pSKH016 |
pBAD322Cm encoding B. subtilis 66-325 |
This study |
pSKH017 |
pBAD322Cm encoding B. subtilis 75-325 |
This study |
pSKH018 |
pBAD322Cm encoding B. subtilis 82-325 |
This study |
pSKH019 |
pET19b encoding N-terminal hexahistidine tagged BirA 64-325, AmpR |
This study |
pSKH020 |
pET19b encoding N-terminal hexahistidine tagged BirA 66-325, AmpR |
This study |
pSKH021 |
pET19b encoding N-terminal hexahistidine tagged BirA 75-325, AmpR |
This study |
pSKH022 |
pET19b encoding N-terminal hexahistidine tagged BirA 82-325, AmpR |
This study |
pMUTIN4 |
spoVG-lacZ, ermR |
[49]
|
pDR111 |
SpcR, 5'-amyE, 3'-amyE, phyper-spank
|
G. W. Ordal |
pSKH023 |
pMUTIN4 encoding B. subtilis bioW internal 500 bp fragment |
This study |
pSKH024 |
pDR111 encoding B. subtilis accB-86
|
This study |