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. 2014 May 9;9(5):e96757. doi: 10.1371/journal.pone.0096757

Table 3. Oligonucleotides utilized.

Oligo-nucleotide Description Sequence
SKH001 BirA F NdeI GAGTGGCTGAACATATGCGGTCAAC
SKH002 BirA stop R Xhol GGCTTGTACCCTCGAGTTAGCCCAATTC
SKH005 accB F NcoI CCATGGAAAAGCAAGATGAGAATCTGCATAAA
SKH006 accB stop R XhoI CTCGAGCTTACTCCGCTTTTACAAGAAATAGAGG
SKH007 pyc F NcoI CCATGGAACGGACAAATCCAAGCCAC
SKH009 pyc stop R XhoI CTCGAGTTTATGCTTTTTCAATTTCAAGGAGC
SKH014 bioO F GATCCTTTCTTCTATTGACAGAAAC
SKH015 bioO R CGC CCTTTCACTGATAACTGAAGAAC
SKH016 yhfU F ACCATCAAAAACCGGTCTGCCATAC
SKH017 yhfU R CCAAAAAGTAATCAAATATGGTTATAC
SKH018 yuiG F ATTGATCGGACTGTCTTGTT
SKH019 yuiG R CCCTTAGGTTGACATACACA
SKH026 EC bioO F GATATGGCGTTGGTCAAAGGCAAG
SKH027 EC bioO R GGGGCTTCTCCAAAACGTGTTTTTTG
SKH034 bioO half F GAAAAAGACCGTTTTGTGTG
SKH035 bioO half R ATTCAAAGGTTAACAATTAGAATATATTATTCTCTCCTG
SKH028 Non 125 F GCATGACGGTTAGCATACAAATGGCAG
SKH029 Non 125 R AACGATCGGGATTTCCATTTTCATCGATTC
SKH036 BirA 64-325 EcoRI F ACTGCGAATTCACCATGAAACCCGGAAAACTCAGTGAAAGCG
SKH037 BirA SalI R ACTGAGTCGACTTAGCCCAATTCGATATCGGCAG
SKH038 BirA EcoRI F CTGACGAATTCACCATGCGGTCAACATTAAGAAAAGACC
SKH040 BirA 64-325 NdeI F CAGTCCATATGAAACCCGGAAAACTCAGTGAAAG
SKH041 BirA BamHI R ACTGGGATCCTTAGCCCAATTCGATATCG
SKH042 EC BirA 65-321 EcoRI F ACTGCGAATTCACCATGCAGTTACTTAATGCTAAACAGATATTG
SKH043 EC BirA SalI R CAGTCGTCGACTTATTTTTCTGCACTACGCAG
SKH044 BirA 82-325 NdeI F ACGTACATATGGGCCAGCATCTTATTTACCATG
SKH045 BirA 82-325 EcoRI F AGCTAGAATTCACCATGGGCCAGCATCTTATTTAC
SKH046 BirA 66-325 EcoRI F ACTGCGAATTCACCATGGGAAAACTCAGTGAAAGCG
SKH047 BirA 66-325 NdeI F CAGTCCATATGGGAAAACTCAGTGAAAGCG
SKH048 BirA 75-325 EcoRI F ACTGCGAATTCACCATGTTTGGATTAAAAACGGAAG
SKH049 EC BirA EcoRI F ACTGGAATTCACCATGAAGGATAACACCGTGCCAC
SKH050 BirA 75-325 NdeI F CAGTCCATATGTTTGGATTAAAAACGGAAG
SKH057 bioW 500 F ACGTGAATTCCATACAGTCAATGCTTTATTAG
SKH058 bioW 500 R GACTGGATCCCTTACCCGCAACATAGCCTG
SKH065 ycgB F CTTACAGAAGAGCGGTAAAAGAAGAAATAAAAAAG
SKH066 lacI R CCGTCTCACTGGTGAAAAGAAAAAC
SKH067 ldH R CATTGCTTTTTCTTTATTTACATCAATGACCACAA
SKH069 RBS accb-86 F ACGGTCGACAAGGAGGAAAAAATATGGAAGCACCAAAGCAAGATG
SKH070 accb-86 R GACGCATGCTTACTCCGCTTTTACAAGAAATAGAGGTTGTC
SKH073 pDR111 F Seq CTCGAGGGTAAATGTGAGCACTCAC
SKH074 pDR111 R Seq GAAAGTATTACATATGTAAGATTTAAATGCAACCG
SKH075 specR F TGAATCTTCTCCATTAGAACATAGGGAGAG
MM1 5' BioW F CGA TCC TTT CTT CTA TTG ACA GAA ACA GG
MM2 LacZ R GGT GTA GAT GGG CGC ATC GTA AC
MM3 pSPAC F CTA CAC AGC CCA GTC CAG ACT ATT CGG
MM4 3' BioW R ATG GCG TCA TCT AGT TCT TTT TTG CGG