Table 2. The effect of different active extracts on cell viability, cellular antioxidant enzymes (Cu/Zn-SOD, Mn-SOD, GSH-Px and CAT) activities and MDA content in MRC-5 cells subjected to H2O2-induced oxidative stress.
Cell viability (%) | Cu/Zn-SOD (U/mL) | Mn-SOD (U/mL) | GSH-Px (U/mL) | CAT (U/mL) | MDA (µM) | |
Control | 100.00±0.552a | 16.138±0.074a | 6.703±1.411a | 17.143±1.276a | 5.199±0.077a | 1.667±0.112f |
H2O2 | 57.247±0.288g | 7.469±0.222g | 0.999±0.428d | 3.158±0.638d | 0.248±0.032e | 8.889±0.224a |
VE+H2O2 | 68.726±0.521e | 8.826±0.369f | 4.706±0.652bc | 9.925±1.276c | 2.010±0.160d | 5.316±0.112c |
CF-AME+H2O2 | 67.058±0.185f | 9.558±2.221e | 5.014±0.521b | 5.414±1.276d | 1.852±0.064d | 7.222±0.114b |
A1+H2O2 | 83.509±0.236b | 12.691±0.074b | 3.259±0.019c | 13.083±0.638b | 3.568±0.064b | 3.730±0.112e |
E1+H2O2 | 77.669±0.352c | 12.012±0.148c | 4.998±0.316b | 10.827±1.276bc | 3.500±0.032b | 4.841±0.337d |
TS#(A1+CF-AME+H2O2) | 75.283±0.173d | 11.124±0.148d | 4.136±0.270bc | 9.248±0.318c | 2.710±0.001c | 5.476±0.116c |
Data were means ± standard deviation (n = 3).
Different letters within the same column indicated that means were statistically different at P<0.05.
TS: the theoretical sum of the antioxidant enzyme activity or MDA content of respective constituent extract with their proportion being 1:1 in the combinations.