Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Mar 6;13:49. doi: 10.1186/1476-4598-13-49

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2014 Li et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Overexpression of AMPK-β1 inhibits cell proliferation and anchorage-independent growth ability. (A) XTT cell proliferation assay showing that enforced expression of AMPK-β1 in A2780cp (C4, C5 and C11) (P < 0.002) and SKOV3 (C1, C2 and C3) (P < 0.001) clones displaying a 45 to 50% decrease in the cell growth rate compared with the empty vector (V1) and the parental (P) cell control. (B) Focus formation assay showing that the size and number of foci was reduced 2.5- to 3-fold in AMPK-β1 stable clones of A2780cp (C4 and C5) (P < 0.001) cells and 3- to 4-fold in AMPK-β1 stable clones of SKOV3 (C1 and C3) (P < 0.001) cells compared with the vector controls. (C) Soft agar assay revealing that the AMPK-β1 stable clones of A2780cp (C4, C5 and C11) (P < 0.04) and SKOV3 (C1, C2 and C3) (P < 0.05) cells had a 2.5- to 3-fold reduction in the size and number of colonies compared with the control. P: parental. V, V1 or V2: empty vector controls.