Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Mar 6;13:49. doi: 10.1186/1476-4598-13-49

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2014 Li et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Depletion of AMPK-β1 promotes cell proliferation and anchorage-independent growth ability. (A) XTT cell proliferation assay demonstrating that OV2008 (C2, C5 and C32) (P < 0.002) and OVCA433 (C1, C12 and C23) (P < 0.001) clones exhibited a 20-25% reduction in cell proliferation. (B) Focus formation assay showing that all AMPK-β1 stable knockdown clones in A2780cp (C2 and C5) (P < 0.001) and OVCA433 (C1 and C12) (P < 0.001) cells expressed a 2 to 3-fold increase in the number of foci. (C) Soft agar assay showing that knockdown of AMPK-β1 in OV2008 (C2 and C5) (P < 0.03) and OVCA433 (C1 and C12) (P < 0.03) cells had a 4-5-fold increase in the number and size of colonies. P: parental. V, V1 or V2: empty vector controls.