Figure 7. miR-7 reverses EMT through EGFR/AKT and EGFR/ERK1/2 pathway.

(A) HO-8910pm and ES-2 cells were transfected with EGFR siRNA or NC, the protein expression of E-cadherin, CK-18, β-catenin and vimentin were explored by western blotting. (B) HO-8910pm and ES-2 cells were treated with LY294002 (20 umol/l) or U0126 (10 umol/l), AKT and ERK1/2 phosphorylation were analyzed by western blotting. (C) HO-8910pm and ES-2 cells were treated with LY294002 (20 umol/l) or U0126 (10 umol/l), the protein expression of CK-18, β-catenin and vimentin were explored by western blotting. GAPDH was used as an internal control (*P<0.05. **P<0.01).