Table 3. Single cell statistics of cln3 MET3-CLN2 with periodic CLN2 expression (period 
min): experimental and simulation values with three different MET3pr strengths.
| Experiment [7] | Low strength | Medium strength | High strength | |
| MET3pr strength | - | 0.5 | 1 | 4 |
| Mean cycle time (M) | 78.00 min | 75.33 0.23 min |
78.01 0.26 min |
79.29 0.70 min |
| Mean cycle time (D) | 89.00 min | 89.45 0.43 min |
85.95 0.33 min |
85.57 0.58 min |
Mean  (M) |
24.00 min | 28.96 0.20 min |
32.86 0.31 min |
33.96 0.72 min |
Mean  (D) |
31.50 min | 42.93 0.32 min |
40.28 0.23 min |
40.65 0.48 min |
| CV cycle time (M) | 0.15 | 0.13 0.00 |
0.14 0.00 |
0.16 0.01 |
| CV cycle time (D) | 0.16 | 0.24 0.01 |
0.18 0.00 |
0.15 0.01 |
CV  (M) |
0.37 | 0.37 0.01 |
0.38 0.01 |
0.47 0.02 |
CV  (D) |
0.37 | 0.47 0.01 |
0.36 0.01 |
0.35 0.01 |
| # complete cycles | - | 2868 281 |
3369 210 |
3025 480 |
| Cycle failure ratio | - | 0.00 0.00 |
0.00 0.00 |
0.05 0.00 |
| Fitting error | - | 0.19 0.01 |
0.12 0.00 |
0.15 0.00 |
For each MET3pr strength value, the simulation statistics (mean 
standard deviation) are computed from 15 independent realizations. In each realization, eight pedigrees are generated. Each pedigree of cells is initiated by a single daughter (D) or mother (M) cell. Fitting error is defined as 
, where 
and 
are the statistical data points in simulations and experiments, respectively (
: mean cycle time of mothers, 
: mean cycle time of daughters, 
: mean G1 duration of mothers, 
: mean G1 duration of daughters, 
: CV of cycle time among mothers, 
: CV of cycle time among daughters, 
: CV of G1 duration among mothers, 
: CV of G1 duration among daughters, and 
through 
denote the simulation values for the same statistics). MET3pr (MET3 promoter strength) is the transcription rate of MET3-CLN2 divided by the transcription rate from the native CLN2 copy. Since the native copy is regulated by SBF, we normalize the full transcription rate by the time-averaged SBF concentration (0.3) in the simulations with no forced CLN2 expression (cln3). The experimental time window 
min to 
min is used in these simulations. As the results indicate, the medium strength MET3pr mimics experimental conditions the best among the three strengths (comparison between the strengths made in the main text). The number of failed cycles (due to event execution errors listed in Table S9) normalized by the number of complete cycles is the cycle failure ratio. Matlab script to reproduce the mean and CV values (medium MET3pr strength) is provided as File C in File S1.