Skip to main content
. 2014 May 9;9(5):e96909. doi: 10.1371/journal.pone.0096909

Figure 3. Effect of CPZ on ASG II fluorescence in cultured HEK293 cells.

Figure 3

Cultured cells were suspended in Tyrode buffer and loaded with ASG II prior to fluorescence measurements, as described in Materials and Methods. Fluorescence was measured at 535 nm in the presence of 4 mM K+ (A), or in the absence of K+ (B) on the extracellular side. Extracellular Na+ was 140 mM in both cases. Fluorescence, indicative of changes in intracellular Na+ concentration, was measured as a function of time, following treatment of cells with DMSO (black squares), 10 µM CPZ (blue squares), or 30 µM CPZ (red squares).