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. 2014 May 9;9(5):e96878. doi: 10.1371/journal.pone.0096878

Figure 4. Treatment of cancerous and normal breast cells with conditioned medium (CM) alters gene expression.

Figure 4

(A) Gene expression changes in MCF-7 cells treated with CM reveal a 10-fold increase in SNAIL, a 7-fold increase in SLUG, and elimination of detectable E-Cadherin mRNA. The increase in EMT markers is accompanied by a down-regulation of ERα mRNA. (B) After treatment of HMEC grown in monolayer culture with CM, there were changes in expression of EMT markers and increases in IL-1β and the growth factor amphiregulin (AREG), suggesting an important role for paracrine signaling between the tumor microenvironment and adjacent normal epithelium.