Table 3. Quantification of Golgi area and fragmentation.
values indicate strong suppression (≥50% decrease relative to Cog3 or ZW10 depletion, respectively),
moderate suppression, and
strong enhancement.
| Protein(s) Depleted | Apparent Golgi Area (A.U) | Number of Golgi Fragments |
|---|---|---|
| Control | 1000±62 | 3.30±0.10 |
| COG3 | 1226±33 | 20.00±6.00 |
| ZW10 | 3752±126 | 19.85±1.75 |
| Rab6 | 715±120 | 5.85±0.40 |
| BicD1/2 | ||
| BicD1 | 1123±176 | 4.50±0.40 |
| BicD1+COG3 | 862±158 | 10.10±1.30 |
| BicD1+ZW10 | 1525±130 | 6.60±1.00 |
| BicD2 | 575±116 | 2.90±1.00 |
| BicD2+COG3 | 518±111 | 6.70±0.90 |
| BicD2+ZW10 | 671±168 | 5.45±0.70 |
| Myosin IIA | ||
| Myosin IIA | 865±106 | 7.00±1.00 |
| Myosin IIA+COG3 | 1016±22 | 22.50±6.25 |
| Myosin IIA+ZW10 | 1156±62 | 10.00±1.00 |
| Kif1C/Kif5B/Kif20A/Dynein | ||
| Kif1C | 1185±223 | 5.25±0.50 |
| Kif1C+COG3 | 1247±82 | 25.55±2.60 |
| Kif1C+ZW10 | 1602±180 | 19.50±6.20 |
| Kif5B | 1377±195 | 7.10±1.10 |
| Kif5B+COG3 | 1188±209 | 25.70±3.65 |
| Kif5B+ZW10 | 1575±186 | 15.00±0.25 |
| Kif20A | 1119±179 | 23.50±1.00 |
| Kif20A+COG3 | 1453±98 | 43.36±3.00 |
| KIF20A+ZW10 | 1578±70 | 12.45±0.80 |
| Dynein (DHC1) | 1851±106 | 28.40±0.90 |
| Dynein+COG3 | 1770±131 | 35.10±1.30 |
| Dynein+ZW10 | 2559±234 | 41.75±1.06 |
| Golgins | ||
| Golgin-97 | 666±103 | 2.90±0.45 |
| Golgin-97+COG3 | 1580±121 | 11.85±1.20 |
| Golgin-97+ZW10 | 1809±59 | 14.65±1.90 |
| OCRL | 1113±35 | 6.85±0.25 |
| OCRL+COG3 | 1251±236 | 16.10±3.25 |
| OCRL+ZW10 | 2199±137 | 28.20±3.85 |
Quantification of Golgi area and fragmentation based on the distribution of GalNAcT2-GFP fluorescence: Golgi area and fragmentation on an individual cellular basis using deconvolved images to sharpen the distinction between Golgi apparatus and general cytoplasm. Images were then segmented to demarcate the Golgi apparatus based on fluorescence intensity and Golgi area and the number of Golgi fragments was determined using iVision for Mac software. At least 30 cells from 4–5 image fields were analyzed per data point. Data are shown ± standard error of the mean. A.U - Arbitrary unit (1000 units = 4653 pixels).