Fig. 2.

Simulated targeted tracer curves (blue, red, and black) and an untargeted tracer curve (green), prior to noise addition, are presented in a. The blue curves correspond to k₃ = 0.05 min⁻¹, the red to k₃ = 0.3 min⁻¹, and the black to k₃ = 0.5 min⁻¹. The solid targeted tracer curves correspond to k₄ = 0 min⁻¹ (irreversible binding) and the dotted lines to k₄ = 0.1 min⁻¹ (reversible binding). Results from fitting the endothelial marker concentration imaging (EMCI) algorithm to the simulated solid curves (irreversible binding) in a to estimate k₃ over a range of k₃ inputs are presented in b. The blue data represents the results for irreversible binding data simulated with a K₁ = 0.007 min⁻¹ while the red data corresponds to the same data simulated with a K₁ = 0.001 min⁻¹. The dashed line represents the line of identity between estimated and true k₃.