Table 1. Sizes and modality signals of the triple reporter tumors after two weeks of growth.

	MDA-MB-231 triple reporter tumor average (n = 8)	HT-1080 triple reporter tumor average (n = 2)
Size measured by caliper (mm3)	96.2±42.1	339.0±63.6
Size measured by PET-CT (mm3)	118.0±57.4	483.5±52.3
Total fluorescence [(p/s)/(cm2/sr)]	3.6×108±1.4×108	1.6×109±5.1×108
Total bioluminescence (p/s)	3.0×108±1.8×108	2.7×1010±8.8×109
Total PET (% bioavailable dose)	9.8×10−2±6.8×10−2	1.7×10−1±5.9×10−2

The tumor size, fluorescence signal, bioluminescence signal, and PET signal were quantified for the MDA-MB-231 and HT-1080 triple reporter tumors expressing all three modalities in vivo. Only tumors that were healthy and large enough to be detectable by all three modalities of the triple reporter construct were averaged and included in this dataset.