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Platelets changed levels of TGF-β1, MMP-2,9, and PAI-1. TGF-β1 levels were measured by ELISA in plasma (A), microdialysates (B), and tumor hemogenates (C) as described in Materials and Methods. (D). Gel zymography analysis of MMP-2, 9 from microdialysates in tumors from PLT-depleted, control mice. (E). Western blot analysis of PAI-1 expression in tumors from PLT-depleted, control mice (normalized to β-actin). The results are expressed as the mean ± SEM. * p < 0.05.
