Table 3.
Summary of oligodendrocytic and astrocytic changes in the anterior horns of mSOD1-Tg mice
| |
12 weeks of age (presymptomatic stage) |
|
18 weeks of age (disease-progressive stage) |
|
20 weeks of age (end stage) |
||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Immunohistochemistry ( n = 5) | qIB ( n = 3) | qPCR ( n = 3) | Immunohistochemistry ( n = 7) | qIB ( n = 3) | qPCR ( n = 3) | Immunohistochemistry ( n = 5) | qIB ( n = 5) | qPCR ( n = 3) | |||
| Cx32 |
Unchanged |
Unchanged |
Unchanged |
|
Decreased |
Decreased |
Unchanged |
|
Decreased |
Decreased |
Decreased |
| Cx47 |
Unchanged |
Unchanged |
Unchanged |
|
Decreased (internalized) |
Unchanged |
Decreased |
|
Decreased (internalized) |
Decreased |
Unchanged |
| Cx30 |
Unchanged |
Unchanged |
Unchanged |
|
Unchanged |
Unchanged |
Unchanged |
|
Unchanged |
Unchanged |
Unchanged |
| Cx43 |
Unchanged |
Unchanged |
Increased |
|
Increased |
Unchanged |
Unchanged |
|
Increased |
Unchanged |
Increased |
| GFAP |
Unchanged |
Unchanged |
Not done |
|
Increased |
Increased |
Not done |
|
Increased |
Increased |
Not done |
| EAAT2 |
Unchanged |
Unchanged |
Not done |
|
Decreased (3/7) |
Decreased |
Not done |
|
Decreased (2/5) |
Decreased |
Not done |
| MOG | Unchanged | Unchanged | Not done | Unchanged | Unchanged | Not done | Unchanged | Unchanged | Not done | ||
Cx, connexin; EAAT2, excitatory amino acid transporter-2; GFAP, glial fibrillary acidic protein; MOG, myelin-oligodendrocyte glycoprotein; qIB, quantitative immunoblot; qPCR, quantitative real-time polymerase chain reaction.