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. 2014 Feb 17;14:99. doi: 10.1186/1471-2407-14-99

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Copyright © 2014 Neiva et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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STAT3 phosphorylation induced by endothelial cell-secreted factors is independent of Akt and ERK phosphorylation. Western blot for phosphorylated and total STAT3, Akt, and ERK in HeLa serum-starved overnight and exposed to A, HDMEC conditioned medium (CM) or unconditioned medium (EBM) for the indicated time points. In addition, HeLa were pre-incubated for 1 to 2 hours with B, 20 μM Stattic; C, 20 μM LY294002; or D, 20 μM U0126, and then exposed to HDMEC CM or EBM in presence of the specific inhibitor for the indicated time points.