Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Feb 17;14:99. doi: 10.1186/1471-2407-14-99

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2014 Neiva et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

STAT3 phosphorylation in xenograft human oral squamous cell carcinomas correlates with tumor cell proliferation and presence of blood vessels. A, HeLa cells were serum-starved overnight and exposed to 20 ng/ml rhIL-6 for the indicated time points. Phosphorylated and total levels of STAT3, Akt, and ERK were determined by Western blots. B-D, xenograft human tumors were generated in SCID mice by co-implanting HeLa and HDMEC. Tumors were retrieved after 28 days, and tissues were analyzed by immunohistochemistry: B, total STAT3 with cytoplasmic localization, diffused through the tissue; C, phosphorylated STAT3 with nuclear localization, concentrated in the proximity of blood vessels; D, Ki67 with nuclear translocation, localized primarily around blood vessels. Photomicrographs at 200×.