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. 2013 Sep 21;13:73. doi: 10.1186/1472-6750-13-73

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Copyright © 2013 Li et al.; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Time course for efficiency of β-glucosidase on lactose in milk and analyzed by HPLC. A. Lactose hydrolysis was measured as a function of time using 311 U/ml β-glucosidase enzyme at 65°C ranging from 5 min to 30 min. All data are presented as the mean ± SD of triplicate determinations. B. HPLC analysis of the reactions performed at 65°C using 311 U/ml β-glucosidase enzyme for 0 min (Panel 1), 10 min (Panel 2), 20 min (Panel 3), or 30 min (Panel 4). Lac = lactose, Glc = glucose, Gal = galactose.