Table 2.
Summary of Antibodies Used in Western Immunoblot (WB) or in Immunohistochemical (IHC) Analysis.
| Antibody | Primary or Secondary | Monoclonal or Polyclonal | Dilution | WB and/or IHC | Source | Company |
|---|---|---|---|---|---|---|
| MMP12 | Primary | Polyclonal | 1:2000 | WB, IHC | Chicken | Abcam, Cambridge, Massachusetts (cat# ab1827) |
| TIMP 1 | Primary | Polyclonal | 1:1000 | WB | Mouse | RDI (Fitzgerald Industries, Concord, Massachusetts (cat# 10R-T112a) |
| TIMP1 | Primary | Monoclonal | 1:100 | IHC | Mouse | Abcam, Cambridge, Massachusetts (cat# ab1828) |
| TIMP2 | Primary | Monoclonal | 1:500 | WB, IHC | Mouse | Abcam, Cambridge, Massachusetts (cat# ab39206) |
| TIMP3 | Primary | Polyclonal | 1:1000 | WB, IHC | Rabbit | Abcam, Cambridge, Massachusetts (cat# ab39206) |
| TIMP4 | Primary | Polyclonal | 1:500 | WB, IHC | Rabbit | Abcam, Cambridge, Massachusetts (cat# ab51207) |
| ADAMTS2 | Primary | Monoclonal | 1:200 | WB, IHC | Mouse | Abcam, Cambridge, Massachusetts (cat# ab56735) |
| β-actin | Primary | Polyclonal | 1:1000 | WB | Rabbit | BioVision, Mountain View, California (cat# 3662-100) |
| α-SM actin | Primary | Polyclonal | 1:50 | IHC | Rabbit | Dako, Carpinteria, California, M0851 |
| HRP-conjugated anti-rabbit IgG | Secondary | Polyclonal | 1:3000 | WB | Donkey | GE Healthcare, Buckinghamshire, United Kingdom |
| HRP-conjugated anti-chicken IgG | Secondary | Polyclonal | 1:10000 | WB | Rabbit | Jackson ImmunoResearch Laboratories, West Grove, Pennsylvania |
| HRP-conjugated anti-mouse IgG | Secondary | Polyclonal | 1:4000 | WB | Sheep | GE Healthcare, Buckinghamshire, United Kingdom |
| Anti-mouse IgG | Secondary | Polyclonal | 1:200 | IHC | Horse | Vector Lab-BA2000 |
| Anti-rabbit IgG | Secondary | Polyclonal | 1:200 | IHC | Goat | Vector Lab-BA1000 |
| Anti-chicken IgY | Secondary | Polyclonal | 1:1000 | IHC | Rabbit | Jackson ImmunoResearch |
Abbreviations: ADAMT, A Disintegrin And Metalloproteinase with Thrombospondin Motifs; Ig, immunoglobulin; HRP, horseradish peroxidase; MMP, matrix metalloproteinase; TIMP, tissue inhibitors of metalloproteinases.