Figure 3.

Bone morphogenetic protein (BMP) 6 suppressed secretory leukocyte peptidase inhibitor (SLPI) and whey acid protein (WAP) 14 mRNA expression in cultured human GC. Cultured human granulosa-lutein cells (GCs) were stimulated with BMP-6 (100 ng/mL) for 24 hours. Total RNA was extracted from the cells and subjected to real-time PCR to determine the expression of SLPI and WAP14. Data were normalized by GAPDH mRNA levels to show the relative abundance. Data from 4 different experiments were combined and shown as the mean ± SEM relative to an adjusted value of 1.0 for the mean value of the each control. *P < .05 (vs control). PCR indicates polymerase chain reaction; mRNA, messenger RNA; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; PCR, polymerase chain reaction; SEM, standard error of mean.