Table 1.
MLVA and MST genotypes of C. burnetii specimens detected from cattle, sheep and a human clinical sample in Hungary
| Sample ID | Source | Town of origin | Year | Ct |
MLVA-6 |
MLVA type | MST type | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Ms23 | Ms24 | Ms27 | Ms28 | Ms33 | Ms34 | |||||||
| Coxi-14 |
cow milk |
Martonvásár |
2010 |
32.90 |
6 |
13 |
2 |
7 |
9 |
9 |
I |
ST20 |
| VS41 |
cow cotyledon |
Nagyecsed |
2012 |
27.78 |
6 |
13 |
2 |
7 |
9 |
9 |
I |
ST20 |
| VS76 |
cow cotyledon |
Szombathely |
2012 |
14.35 |
6 |
13 |
2 |
7 |
9 |
10 |
J |
ST20 |
| VS79 |
cow cotyledon |
Nyírbátor |
2012 |
30.34 |
6 |
13 |
2 |
7 |
9 |
10 |
J |
ST20 |
| VS93 |
cow cotyledon |
Berkesd |
2012 |
15.39 |
6 |
13 |
2 |
7 |
9 |
11 |
M |
ST20 |
| VS103 |
cow cotyledon |
Kapuvár |
2012 |
16.89 |
6 |
13 |
2 |
7 |
9 |
9 |
I |
ST20 |
| VS16 |
sheep cotyledon |
Balkány |
2012 |
8.85 |
0 |
8 |
4 |
5 |
6 |
2 |
AF |
ST37 |
| VS27 |
sheep cotyledon |
Jászfelsőszentgyörgy |
2012 |
29.83 |
0 |
8 |
4 |
5 |
6 |
2 |
AF |
ST37 |
| VS38 |
sheep cotyledon |
Beremend |
2012 |
12.79 |
0 |
8 |
4 |
5 |
6 |
2 |
AF |
ST37 |
| VS42 |
sheep cotyledon |
Kunmadaras |
2012 |
30.72 |
0 |
8 |
4 |
5 |
6 |
2 |
AF |
ST37 |
| VS108 |
sheep cotyledon |
Biharnagybajom |
2013 |
8.73 |
0 |
9 |
5 |
5 |
6 |
2 |
AG |
ST28 |
| 4756 |
human blood |
Hortobágy |
2011 |
28.31 |
5 |
9 |
5 |
ND |
6 |
2 |
partial |
ND |
| C.burnetii RSA493* | tick | USA | 1935 | 9 | 27 | 4 | 6 | 9 | 5 | ST16 | ||
Ct: cycle threshold value of real-time PCR assay targeting the multicopy IS1111 insertion element of the C. burnetii genome [22] to quantify the approximate bacterial load, 0: no amplification, ND: not determined probably because of insufficient DNA content, *: Genotype of RSA493 strain (Nine Mile, GenBank accession number: AE016828) was determined by in silico analysis.