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. 2014 Apr 29;12:132. doi: 10.1186/1477-7819-12-132

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Copyright © 2014 Wu et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Immunohistochemical labeling of lymphatic microvessels (EnVision™) and LMVD in primary gastric cancer tissue and noncancerous gastric tissue. (A-D) Lymphatic microvessels labeled with D2-40 by IHC (EnVision™). (A, B) Gastric cancer (×100), (A) peritumoral lymphatic vessels were large, within which was nests of no staining tumor cells (asterisks); (B) intratumoral lymphatic vessels had narrow and collapsed lumen, with irregular cell walls (long arrow). (C) Invaded muscle tissue (×200). There were a few tumor cells in dilated lymphatic microvessels, as ‘lymphatic vessel invasion’ (asterisks). (D) Non-cancerous mucosa (×200). Long arrows indicated lymphatic microvessels, and short arrows denoted blood capillaries. Asterisks represented the areas where tumor cells are. (E) LMVD in gastric cancer tissue (T) was statistically higher than that in the non-cancerous counterparts (N) (*** P <0.001).