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EhCRT-human C1q on the activation of classical complement pathway: hemolysis assay. 1: corresponding to NHS (positive control); 2: human C1q-depleted serum (NHSC1q−); 3: (NHSC1q−) + C1q; 4: (NHSC1q−) + C1q + EhCRT; 5: (NHSC1q−) + C1q + EhCRT + IgG anti-EhCRT. Assays were performed in triplicate; values are the mean of three different experiments ± SD. Differences between groups ∗, ●, and ▼ were compared through ANOVA test. Statistical significance (P = 0.012).
