Figure 2.

Inhibition of bovine trypsin and total proteolytic activity of HGPs from pigeon-pea fed larvae by BmPI: the assays were conducted using azocasein as substrate. The experiments were done in triplicates and mean was calculated. *IC₅₀ value of BmPI against bovine trypsin was 2.68 µg/mL; **IC₅₀ value against total HGPs was 2 µg/mL. IC₅₀ is concentration of inhibitor, which reduces the enzyme activity to 50% of the original.