Different expressions of VM-related molecules in HNSCC patients between the VM-positive group and VM-negative group ((a), (b), (c), and (d)). (a) Different mRNA expressions of EphA2 (P = 0.023), E-cadherin (P = 0.035), claudin4 (P = 0.034), DSG-3 (P = 0.022), Twist (P = 0.001), and Vimentin (P = 0.024) between the VM-positive group and VM-negative group. (b) Different protein expressions of EphA2 (P = 0.004), E-cadherin (P = 0.044), claudin4 (P = 0.006), and DSG-3 (P = 0.032) between the VM-positive group and VM-negative group by immunohistochemistry. (c) Different protein expressions of Twist (P = 0.039) and Vimentin (P = 0.044) between the VM-positive group and VM-negative group by immunohistochemistry. (d) By immunohistochemistry, EphA2, E-cadherin, claudin4, DSG-3, and Vimentin were found to be expressed in cytoplasm of HNSCC cells. Twist was expressed mainly in the nucleus.