Figure 2.

Histochemical tests in the longitudinal section of seeds of Libidibia ferrea. (a) Seed control showing the organization of main issues. (b) Sudan IV test that revealed the presence of lipids in the cotyledon and (c) in the seed coat cuticle. (d) Osmium tetroxide test showed that the lipids are mainly unsaturated and predominantly observed in the cotyledon, (e) in the basal portion of macrosclereids, and (f) in the fibers. (g-h) Nile blue sulphate test reveals the presence of neutral and acids lipids in the cuticle epidermis (open triangle) of cotyledons and (i) mainly in the subcuticular space (asterisks). (j) NADI reagent also revealed the presence of terpenoid only in the epidermal cells of cotyledons (arrows). ms: macrosclereid; os: osteosclereids; fi: fibers; pa: parenchyma; co: cotyledon; ep: epidermis of cotyledon; se: subepidermis of cotyledon; sc: subcuticular space. Scale bar: (a, g) = 200 μm; (b-c, j) = 20 μm; (d-e) = 100 μm; (f, i) = 50 μm; (h) = 25 μm; (j) = 40 μm.