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The effects of different antagonists on the antinociceptive activity of the LPLF in the acetic acid-induced contortions. Animals were pretreated with naloxone (5 mg/kg, i.p.), atropine (5 mg/kg, i.p.), or celecoxib (5 mg/kg, i.p.) 30 min prior to oral administration of the Libidibia ferrea seed oil (10 mg/kg, p.o.). The results are presented as the mean ± S.E.M (n = 6) of total writhings. ***P < 0.01 compared to control group, Tukey's multiple comparison test.
