Figure 7. p27 knockdown partially rescues MLN4924-induced migration and proliferation, but not tube formation.
MS1 cells were first transfected with siRNA targeting p27 and si-Cont for 72 hrs, followed by MLN4924 treatment for 24 hrs. One portion was subjected to IB (A), and the other portion was used for assays of migration (B), proliferation (C) and tube formation (D). Shown is mean ± SEM from three independent experiments. Scale bar represents 50 μm.