Salicylic acid (SA) potentiates flg22-induced ROS burst.
Two-week-old Col-0, atrbohD, npr1, and NahG seedlings were pre-treated with SA (100 μM) or equal volume of DMSO solvent for two days, and then treated with flg22 (30 nM). ROS burst was monitored by luminol-based ROS detection using a photon-counting camera. Error bars indicate SD (n = 3). Single and double asterisks above the data points indicate differences that are statistically significant (p<0.05) and statistically very significant (p<0.01), respectively.
(a) Enhance ROS burst by SA pre-treatment is dependent on functional AtRbohD gene.
(b) SA-potentiation of ROS burst is dependent on NPR1.
(c) Enhanced ROS burst in seedlings with SA pretreatment is abolished in NahG background.