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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Plant J. 2013 Dec 17;77(2):222–234. doi: 10.1111/tpj.12382

Figure 5.

Figure 5

Salicylic acid (SA) potentiates flg22-induced ROS burst.

Two-week-old Col-0, atrbohD, npr1, and NahG seedlings were pre-treated with SA (100 μM) or equal volume of DMSO solvent for two days, and then treated with flg22 (30 nM). ROS burst was monitored by luminol-based ROS detection using a photon-counting camera. Error bars indicate SD (n = 3). Single and double asterisks above the data points indicate differences that are statistically significant (p<0.05) and statistically very significant (p<0.01), respectively.

(a) Enhance ROS burst by SA pre-treatment is dependent on functional AtRbohD gene.

(b) SA-potentiation of ROS burst is dependent on NPR1.

(c) Enhanced ROS burst in seedlings with SA pretreatment is abolished in NahG background.