Table 2.
Differential glycopattern in gastric ulcer and cancer according to the lectin microarray analysis based on data from 37 lectins giving significant signals
| Lectin | Specificity |
Normalized fluorescent intensity1 |
Ratio, P value | |
| Gastric cancer | Gastric ulcer | (C/U) | ||
| Jacalin | Galβ1-3GalNAcα-Ser/Thr(T) and GalNACα-Ser/Thr(Tn) | 0.49 | - | P < 0.001 |
| ECA | Galβ-1,4GlcNAc | 0.72 | - | P < 0.001 |
| HHL | Polymannose (α-1,3) and (α-1,6) linked mannose | 0.64 | - | P < 0.001 |
| WFA | GalNAcα/β-1,3/6Gal | 0.64 | - | P < 0.001 |
| GSL-II | GlcNAc | 0.67 | - | P < 0.001 |
| MAL-II | Sia2-3Galβ1-4Glc (NAc) | - | - | / |
| PHA-E | Bisecting GlcNAc and biantennary N-glycans | 0.65 | - | P < 0.001 |
| PTL-I | αGalNAc | 0.67 | - | P < 0.001 |
| SJA | Terminal in GalNAc and Gal | 0.12 | 0.38 | 0.32, P < 0.001 |
| PNA | Galβ1-3GalNAcα-Ser/Thr(T) | 0.08 | 0.22 | 0.36, P < 0.001 |
| EEL | Galα1-3(Fucα1-2)Gal | 1 | 0.23 | 4.41, P < 0.001 |
| AAL | Fucα-1,6GlcNAc, Fucα-1,3LacNAc | 0.89 | - | P < 0.001 |
| LTL | α-L-Fuc | 0.59 | 0.31 | / |
| MPL | αGalNAc | 0.69 | - | P < 0.001 |
| LEL | Poly-LacNAc and (GlcNAc)n | 0.54 | 0.27 | / |
| GSL-I | αGalNAc, αGal | - | 0.03 | P < 0.001 |
| DBA | GalNAcα-Ser/Thr(Tn) | 0.4 | 0.2 | / |
| LCA | α-Man and Fucα-1,6GlcNAc (core fucose) | 0.61 | - | P < 0.001 |
| RCA120 | Gal and GalNAc | 0.61 | 0.17 | 3.55, P < 0.001 |
| STL | (GlcNAc)n | 0.54 | 0.31 | / |
| BS-I | αGal and αGalNAc | 0.12 | - | P < 0.001 |
| ConA | α-Man (inhibited by presence of bisecting GlcNAc) | 0.13 | 0.11 | / |
| PTL-II | Gal | 0.66 | - | P < 0.001 |
| DSA | β1-4GlcNAc and LacNAc | 0.79 | 1 | 0.79, P < 0.001 |
| SBA | Terminal GalNAc (especially GalNAcα1-3Gal) | 0.38 | - | P < 0.001 |
| VVA | GalNAc and GalNAcα-Ser/Thr(Tn) | 0.45 | - | P < 0.001 |
| NPL | Polymannose(α-1,6)linked mannose | 0.64 | 0.12 | 5.21, P < 0.001 |
| PSA | Fucα-N-acetylchitobiose -man | 0.85 | 0.2 | 4.19, P < 0.001 |
| ACA | Galβ1-3GalNAcα-Ser/Thr(Tn) | 0.71 | 0.18 | 3.84, P < 0.001 |
| WGA | Terminal GlcNAc and (GlcNAc)n | 0.35 | - | P < 0.001 |
| UEA-I | Fucoseα1-2Galβ1-4Glc(NAc) | 0.81 | - | P < 0.001 |
| PWM | GlcNAc | 0.8 | - | P < 0.001 |
| MAL-I | Galβ-1,4GlcNAc and Sia2-3Galβ1-4Glc(NAc) | 0.63 | 0.16 | 3.89, P < 0.001 |
| GNA | (α-1,3)Man | 0.78 | 0.09 | 9.1, P < 0.001 |
| BPL | Galβ1-3GalNAc | 0.67 | - | P < 0.001 |
| PHA-E+L | Tri-and tetra-antennary complex-type N-glycan | 0.92 | - | P < 0.001 |
| SNA | Sia2-6Galβ1-4Glc(NAc) | 0.61 | 0.18 | 3.39, P < 0.001 |
1
Signal intensities obtained from nine replicate blocks on three replicate slides were normalized and averaged, and the ratio of gastric cancer vs gastric ulcer (C/U) was calculated. -: Negative signal; /: No significant difference.