Figure 2.

Overview of functional profiling in yeast. About 4600 deletion strains uniquely identified by DNA sequences (barcodes) are pooled and exposed to a toxicant at multiple doses and generation times (5 or 15). Barcodes are amplified from purified genomic DNA by PCR and counted by hybridization to a microarray or high-throughput sequencing methods. Subsequent analyses of individual strains can confirm susceptibility or resistance to the toxicant.