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. Author manuscript; available in PMC: 2014 May 12.
Published in final edited form as: Pharm Res. 2011 Dec 3;29(6):1506–1517. doi: 10.1007/s11095-011-0625-0

Fig.3.

Fig.3

Effect of apigenin intake on the extent of proliferation in the dorso-lateral prostates of TRAMP mice. A Apigenin intake in TRAMP mice at 20 and 50µg/day exhibited decreased nuclear expression of proliferating cell nuclear antigen (PCNA) in a dose-dependent manner as ascertained by immunohistochemical analyses. In vehicle-treated TRAMP mice, extensive PCNA staining was observed in the nuclei of epithelial cells compared with non-transgenic mice. B Statistical box plot analysis of PCNA nuclear presence was performed by counting PCNA positive nuclear stained cells from various locations in non-transgenic control, TRAMP control, TRAMP (20µg/day apigenin) and TRAMP (50µg/day apigenin). Box plot for positive nuclei (%), black bar = median, red box = 25th to 75th percentiles, Bars = entire range from 5 mice. Details are described in ‘Materials and Method’ section.