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. Author manuscript; available in PMC: 2014 May 12.
Published in final edited form as: Pharm Res. 2011 Dec 3;29(6):1506–1517. doi: 10.1007/s11095-011-0625-0

Fig.5.

Fig.5

Effect of apigenin intake on total ERK, p-ERK1/2, total Akt and p-Akt (Ser473), (Thr-308) levels in the dorsolateral prostates of TRAMP mice. A Dorso-lateral prostate tissues levels of phosphorylated-Akt (Ser473), phosphorylated-ERK1 (T202/Y204)/ ERK2 (T185/Y187) were detected through ELISA method as provided by the vendor’s protocol. Apigenin intake caused dose-dependent decrease in p-Akt (Ser473) and p-ERK1/2 expression. **P < 0.001, TRAMP apigenin versus TRAMP control (Kruskal-Wallis test). Bars ± SE of 6 mice B Protein expressions of Akt1/2, p-Akt (Ser473) and (Thr-308); total ERK and p-ERK1/2 in the total lysate of dorsolateral prostates of non-transgenic control, TRAMP control, TRAMP (20µg/day apigenin) and TRAMP (50µg/day apigenin) was detected by Western blotting. A significant decrease in p-Akt (Ser473) and p-ERK1/2 protein expression was observed after apigenin feeding, whereas, Akt1/2, total ERK1/2 and p-AKT (Thr-308) protein expressions were unaffected in all TRAMP groups. Representative data from two mice per group. Equal loading of protein in the lanes was confirmed by stripping the membrane and reprobing it with β-actin, a housekeeping protein. Details are described in ‘Materials and Method’ section.