Figure 1.

Hierarchical cluster analysis of Raman dataset. (A) Raman image reconstructed from the C-H stretching intensity. HCA is performed in the following ranges: (B) 1200–1800 and 2800–3100 cm⁻¹; (C) 1200–1800 cm⁻¹; and (D) 2800–3100 cm⁻¹. The morphology and topology of some clusters suggest that they are associated with the cytoplasm (green), nucleus (blue), nucleoli (brown), and lipid droplets (red). Other clusters in panels B–D cannot be assigned in this manner. (E) The mean cluster Raman spectra are also shown.