Colocalization of the endoplasmic reticulum, Golgi apparatus, and mitochondria. (A1–C1) Fluorescence images of the nucleus (blue), endoplasmic reticulum (A1), Golgi apparatus (B1), and mitochondria (C1). (A2–C2) HCA of CARS datasets. (A3–C3) Overlaid images of endoplasmic reticulum, Golgi apparatus, and mitochondria staining (red) from panels A1–C1 with their corresponding HCA (green) from A2–C2 and the overlapping regions (yellow).