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. 2014 May 6;106(9):1910–1920. doi: 10.1016/j.bpj.2014.03.025

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© 2014 by the Biophysical Society.

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Automatic identification of the nucleus, nucleolus, and lipid droplets. (A) Random forest of CARS dataset. (B) Fluorescence imaging of the nucleus and lipid droplets. Overlaid images of lipid droplets and the nucleus fluorescence (red) from panel B with their corresponding random forest classes (green) from panel A are displayed in panels C (lipid droplets) and D (nucleus), respectively. (yellow) Overlapping region. (D, inset) Overlay region of the nucleolus between the fluorescence (red) and random forest (green).