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. 2014 May 6;106(9):1864–1870. doi: 10.1016/j.bpj.2014.03.016

Figure 1.

Figure 1

Height change of isolated thylakoid membranes in response to PSII-specific illumination. (A) Diagram of the experiment showing isolated thylakoid membranes illuminated and imaged by an inverted microscopy, whereas the height (h) is measured by AFM. The fluorescence image shows reduced punctate structures after PSII-specific illumination (640 nm), indicating that unstacking has occurred. Scale bar = 5 μm. (B) A typical height response from a thylakoid membrane sample exposed to PSII-specific illumination. There is a time delay (td) between the light and the height change of the thylakoid membrane. (C) Average height of 20 thylakoid membranes before and after the PSII-specific illumination. (D) Average of the time delay at different light intensities. (1 μmol photons = 186.6 mJ for 640 nm light) To see this figure in color, go online.