Table 1.
L-type conductance and intramembrane charge movement
|
G-V |
Q-V |
||||||
|---|---|---|---|---|---|---|---|
| Gmax (nS/nF) | V1/2 (mV) | kG (mV) | Vrev (mV) | Qmax (nC/μF) | VQ (mV) | kQ (mV) | |
| Naïve | 124 ± 12 (19) | 0.7 ± 1.4 | 4.9 ± 0.4 | 69.1 ± 2.3 | 25.5 ± 1.7 (35) | −9.7 ± 2.5 | 11.8 ± 0.8 |
| YFP | 120 ± 11 (12) | −0.9 ± 24 | 5.2 ± 0.5 | 66 ± 1.1 | 23.8 ± 2.1 (15) | −11.4 ± 1.8 | 11.0 ± 0.7 |
| V-Rad | 63 ± 10a (9) | −4.2 ± 5.3 | 5.6 ± 0.4 | 60.6 ± 2.0b | 16.4 ± 1.0c (23) | −10.5 ± 2.3 | 12.5 ± 0.9 |
| V-Rem | 76 ± 7a (11) | −1.4 ± 1.8 | 5.7 ± 0.5 | 60.5 ± 2.1b | 23.5 ± 1.2 (22) | −10 ± 2.2 | 10.8 ± 0.6 |
| V-RadNT-Rem | 86 ± 5b (11) | 2.4 ± 1.8 | 6.5 ± 0.2b | 59.2 ± 1.5b | 14.8 ± 1.9a (8) | −3.6 ± 3.4 | 11.4 ± 1.0 |
| V-RemNT-Rad | 66 ± 6a (6) | 7.0 ± 5.0 | 7.3 ± 0.4b | 53.2 ± 2.6c | 22.0 ± 1.7 (9) | −8.4 ± 2.8 | 11.9 ± 1.0 |
| V-RemcoreCT | 77 ± 6b (7) | 8.6 ± 1.7 | 5.1 ± 1.5 | 60.2 ± 4.4 | 21.9 ± 2.8 (6) | −10.7 ± 1.8 | 11.6 ± 1.2 |
Data are given as mean ± SE, with the numbers in parentheses indicating the number of FDB fibers tested. Conductance and charge movement data were fit by Eqs. 1 and 2, respectively. Significant differences between naïve fibers and fibers expressing either YFP or a particular V-RGK construct are indicated.
a
p < 0.005; one-way ANOVA, followed by a Dunnett’s post hoc test.
b
p < 0.05; one-way ANOVA, followed by a Dunnett’s post hoc test.
c
p < 0.001; one-way ANOVA, followed by a Dunnett’s post hoc test.