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. Author manuscript; available in PMC: 2014 May 12.
Published in final edited form as: Free Radic Biol Med. 2010 Dec 21;50(5):592–601. doi: 10.1016/j.freeradbiomed.2010.12.014

Figure 3. FA-OOH does not affect extra-mitochondrial superoxide release during forward electron transfer.

Figure 3

Amplex red was used to measure H2O2 production in the presence of substrates specific for complex I or II and complex I inhibitor, rotenone - (A) glutamate/malate, 5 mM and (B) succinate, 5 mM + rotenone, 0.5 μM. The amplex red buffer was prepared with ± 30 Units/mL of CuZnSOD. FA-OH or FA-OOH was added at a final concentration of 0.75 μM. Statistical significance was assessed by one-way ANOVA with Newman Keul’s multiple comparison test (* p < 0.0001 vs. untreated mitochondria). The results shown represent means ± S.E.M. for 5-6 individual mitochondrial preparations.