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. Author manuscript; available in PMC: 2014 May 12.
Published in final edited form as: Free Radic Biol Med. 2010 Dec 21;50(5):592–601. doi: 10.1016/j.freeradbiomed.2010.12.014

Table 1.

FA-OH and FA-OOH decrease mitochondrial membrane potential during reverse electron transfer.

Subs/Inhibit Untreated FA-OH FA-OOH
G/M 738.83±44.33 704.65±39.55 711.48±37.08
S/R 721.88±45.27 702.68±44.63 692.90±45.93
S 507.73±38.08 326.23±45.27* 309.83±25.34*

Mitochondrial membrane potential was measured using Safranin O (5 μM) in the presence of substrates specific for complex I or II (glutamate/malate (G/M), 5 mM; succinate (S), 5 mM) and inhibitor (rotenone (R), 0.5 μM). FA-OH or FA-OOH was added at a final concentration of 0.75 μM per assay. Fluorescence was measured at excitation/emission wavelengths set at 485 nm/590 nm. Results are expressed as change in fluorescence. The results shown represent means ± S.E.M. for 4 individual mitochondrial preparations. Statistical significance was assessed by one-way ANOVA with Newman Keul’s multiple comparison test

*

(p < 0.0001 vs. untreated mitochondria).

Subs/Inhibit = substrates/inhibitors.