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. Author manuscript; available in PMC: 2014 May 12.
Published in final edited form as: Free Radic Biol Med. 2010 Dec 21;50(5):592–601. doi: 10.1016/j.freeradbiomed.2010.12.014

Table 2.

Extra-mitochondrial superoxide release by isolated skeletal muscle mitochondria respiring on complex I and II-linked substrates (measured by electron paramagnetic resonance).

Subs/Inhibit Untreated FA-OH FA-OOH
G/M 423.44±25.7 394.38±22.7 454.06±27.5
S/R 498.75±57.0 473.75±42.8 425.00±55.8
S 536.67±27.6 501.88±21.6 503.44±10.3

Skeletal muscle mitochondria (20 μg) were incubated with substrates (24 mM glutamate/malate (G/M) or succinate (S)), inhibitor (2.4 μM rotenone (R)) and DIPPMPO (50 mM) for 10 min at 37°C. Fatty acid hydroxide (FA-OH), or fatty acid hydroperoxide (FA-OOH) were added at the final concentration of 0.75 μM. Units are in relative intensity/20 μg protein. Values are in mean ± S.E.M. Statistical significance was assessed by one-way ANOVA with Newman Keul’s multiple comparison test (n = 6). Subs/Inhibit = substrates/inhibitors.