Figure 4.

Treating 5-month-old Ppt1^−/− mice with 10 mg/kg memantine has no effect.

An accelerating rotarod (0-40 rpm in 120 seconds) was used to measure the motor skills of Ppt1^−/− and wild-type (WT) mice. Mice were intraperitoneally injected with the NMDA receptor antagonist memantine (10 mg/kg) or a vehicle control (physiological saline) at 5 months of age and reevaluated on a monthly basis. (A) At 3 time points, Ppt1^−/− mice performed significantly better than wild-type mice. (B) Treatment with 10 mg/kg memantine at 5 months of age does not significantly affect Ppt1^−/− performance. (C) Treatment with 10 mg/kg memantine at 5 months of age does not have a significant effect on wild-type mice. Panel D is an overlay of the previous data sets. Data points represent mean ± S.E.M. of the time mice were able to stay on the rod. Two-way ANOVA with Sidak’s posttest for multiple comparisons was used to compare vehicle-treated wild-type mice to other conditions and to compare treated and untreated Ppt1^−/− populations (*P < 0.05, ***P < 0.001). Wild-type vehicle, n = 6; Ppt1^−/− vehicle, n = 8; wild-type memantine, n = 6; Ppt1^−/− memantine, n = 7.