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. Author manuscript; available in PMC: 2014 May 12.
Published in final edited form as: Biochim Biophys Acta. 2012 Jun 12;1820(10):1519–1525. doi: 10.1016/j.bbagen.2012.06.002

Fig. 3.

Fig. 3

Single-cycle SPR kinetic analysis of HypA–UreE binding. Analyte concentrations of 6.25, 12.5, 25, 50 or 100 nM UreE (as shown by arrows) were injected across a CAP chip with 214 RU tethered biotinylated HypA. Association times were 120 s and were followed by dissociation phases of 180 s prior to the next injection of analyte. Raw data are shown with an overlapping fit to a two-state sequential binding model.