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. Author manuscript; available in PMC: 2014 May 12.
Published in final edited form as: Biochim Biophys Acta. 2012 Jun 12;1820(10):1519–1525. doi: 10.1016/j.bbagen.2012.06.002

Fig. 4.

Fig. 4

BLI analysis of UreG-UreE interactions. A. Binding of ligand UreG to analyte UreE in the absence of zinc. Phases are 0–300 s, association; 300–600 s, dissociation; 600–900 s, dissociation in buffer with 5 mM EDTA. Analyte concentrations were (from bottom to top): 0, 0.312, 0.625, 1.25, 2.5, 5, 10 or 20 µM UreE. B. Binding in the presence of zinc. All conditions and concentrations were the same as in A, except that 1 mM ZnCl2 was added to all phases. C. Association phase in the absence of zinc; fits to two-state parallel model. D. Association phase in the presence of zinc; fits to two-state parallel model. E. Dissociation phase in the absence of zinc; fits to a two-state model. F. Dissociation phase in the presence of zinc; fits to a one-state model with plateaus.