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. Author manuscript; available in PMC: 2014 May 12.
Published in final edited form as: Cell Rep. 2014 Feb 27;6(5):878–891. doi: 10.1016/j.celrep.2014.01.040

Figure 4. Fragmentation of the Golgi by EspG Is PAK Independent.

Figure 4

(A) PAK and Rab1 share the binding surface on EspG. Protein Data Bank structures 3PCS and 4FME were used for alignment.

(B) Binding of EspG constructs to PAK AID. The ARF1-binding deficient mutant (IPP) does not interact with PAK.

(C) Kinase assay showing the sensitivity of PAK activation to IPA-3. Radiography data from the assays were quantified and plotted to determine IC50 values.

(D and E) Golgi fragmentation is induced by EspG in cells treated with IPA-3 to inhibit PAK. Microinjected cells are marked with an asterisk.