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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Nat Biotechnol. 2014 Mar 16;32(4):364–372. doi: 10.1038/nbt.2858

Fig. 3. Monocytes in MI(S)TRG mice are functional.

Fig. 3

a-b, Cytokine production by human monocytes isolated from the BM of MITRG recipients and stimulated overnight in vitro with 100 ng/ml LPS (a) or 10 μg/ml R848 (b) (error bars indicate SD of triplicates; representative of 3 independent experiments) was measured by ELISA. c, In vitro phagocytosis of GFP-expressing E.coli by human cell subsets, identified by flow cytometry gating, in the blood of MITRG mice (n=7). d-f, Mice were injected with LPS (d; 90 min, n=15-18), or infected with Listeria monocytogenes (e; day 2, n=6-15) or influenza A/PR8 H1N1 (f; day 3, n=3-5). Cytokine production was measured by ELISA (in the serum) or by RT-PCR (in the lung). (d) p-value calculated by unpaired Student's t-test on log10-transformed values; (e,f) p-values calculated by one-way ANOVA followed by Tukey posthoc test (* p<0.05).