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. Author manuscript; available in PMC: 2014 May 12.
Published in final edited form as: J Cell Biochem. 2013 Nov;114(11):2612–2623. doi: 10.1002/jcb.24609

Fig. 1.

Fig. 1

TUNEL assay of preconditioned mesenchymal stem cells (MSC) following exposure to hypoxia. A: MSCs exposed only to normoxia without treatment (control). B: MSCs preconditioning with hyperoxia; (C) MSCs preconditioned with pan-caspase inhibitor (CI); and (D) MSCs preconditioned with the combination of hyperoxia and CI prior to hypoxia exposure. Preconditioning with hyperoxia, CI, or the combination resulted in a decrease in apoptosis as compared to (E) MSCs exposed to hypoxia without preconditioning. This is obvious by a decrease in green fluorescence which represents apoptotic MSCs. Nucleic DNA has been labeled by DAPI staining (blue fluorescence). F: The apoptotic score index determined by TUNEL assays for MSCs preconditioned with hyperoxia, CI or the combination are shown. P-values above columns represent comparison to control; NS, non- significant.