Table 1. MutY Activity Assay (CC104 lac+ Forward Reversion Assay).
| strain | plasmida | no. of lac+ revertantsb | relative to CC104c |
|---|---|---|---|
| CC104 | p(empty) | 4.6 ± 0.4 | 1 |
| CC104 ΔdinG | p(empty) | 7.5 ± 0.7 | 1.6d |
| CC104 | p(EndoIII D138A) | 8.5 ± 1.1 | 1 |
| CC104 ΔdinG | p(EndoIII D138A) | 6.7 ± 0.6 | 0.8d |
| CC104 | p(EndoIII Y82A) | 3.4 ± 0.3 | 1 |
| CC104 ΔdinG | p(EndoIII Y82A) | 5.8 ± 0.8 | 1.7d |
a
See Table S2 for full plasmid designation.
b
Values for lac+ revertants per 109 cells are reported for at least three independent trials (N ≥ 20) ± SEM.
c
Relative to CC104 is defined as the ratio of lac+ revertants for the CC104 dinG knockout strain to the number of lac+ revertants for the wild-type CC104 strain containing the same plasmid as the CC104 dinG knockout strain
d
(p < 0.01)