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Reactions of C165A AhpC-SOH (40 μM) with 1 or 4 (100 μM) at rt in 50 mM NH4HCO3, pH 7.5. (A) ESI-TOF mass spectrum of the AhpC-SOH reaction with 4 at 30 min. (B) Time course of adduct formation. The concentration of AhpC-SOH adduct with 1 or 4 along the reaction time course was determined based on relative abundance of adduct among the total ion abundances of the prominent species in the ESI-TOF mass spectra.
