Figure 4.

Reactivity of 4 with oxidized cell lysates. (A) Reduced protein lysates were spiked with C165A AhpC for loading control and aliquotted into 60 μg fractions. Fractions were treated with 4 (100 μM) and increasing concentrations of H₂O₂. A control sample of lysate (lane 2) was supplemented with TCEP then treated with 4 (100 μM). (B) Reduced protein lysate (60 μg fraction) was treated with iodoacetyl biotin (100 μM) to label all thiol content and compared to the sample in (A) treated with 5 μM H₂O₂ and 100 μM of 4.